TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 Liters
CVE 17884
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High quality TRIS Acetate EDTA 10X Solution for research or manufacturing use
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O que se Destaca
Detalhes do produto
- Product is for research or further manufacturing use only, not for food or drug use
- Common Name: TRIS Acetate EDTA 10X Solution
- Substance Name: TRIS Acetate EDTA 10X Solution
- Soluble in: Water
- Store at: Room Temperature
- 10X solution contains: TRIS-Base: 0.4 M Acetic Acid: 0.2 M EDTA: 10 mM pH (10X Solution (@25°C): 8.2-8.4
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 4.2 pounds (1.91 kg) |
| Volume | 4 liters |
| Brand Name | RPI |
| Manufacturer | RPI, Corp. |
| Part Number | T60010-4000.0 |
| Brand | RPI |
| Item Volume | 4 liters |
| Package Information | Bottle |
Quem Deverá Comprar?
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Molecular Biologists
Ideal for molecular biology experiments requiring precise buffering capacity and DNA/RNA stabilization in gel electrophoresis.
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Laboratory Researchers
Perfect for researchers conducting experiments in genetics, proteomics, and biochemistry in a lab environment.
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Educational Institutions
Useful for universities and colleges providing practical lab experiences for students in biological sciences.
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Non-Scientific Use
Not suitable for casual users or applications outside of professional research and laboratory environments.
DESCRIÇÃO DO PRODUTO
TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 Liters
Perguntas e respostas do cliente
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Pergunta:
What is TAE 10X Solution used for?
Resposta: TAE 10X Solution, containing TRIS, acetate, and EDTA, is primarily utilized in molecular biology laboratories for DNA and RNA electrophoresis. Its buffering capacity ensures stable pH during experiments, which is crucial for preserving nucleic acid integrity. Scientists often employ TAE for gel electrophoresis, allowing them to effectively separate and analyze nucleic acid fragments, making it essential for tasks like cloning, restriction digestion analysis, and PCR product verification. -
Pergunta:
How do you prepare TAE 1X Solution from TAE 10X Solution?
Resposta: To prepare TAE 1X Solution from TAE 10X Solution, dilute the 10X concentration by mixing one part of TAE 10X with nine parts of distilled water. This means adding 100 mL of TAE 10X to 900 mL of water, resulting in a total volume of 1 liter of 1X solution. Proper dilution is vital for effective electrophoresis, ensuring optimal running conditions for your gel and preventing any alteration in nucleic acid behavior during analysis. -
Pergunta:
Is TAE 10X Solution suitable for RNA applications?
Resposta: Yes, TAE 10X Solution is suitable for RNA applications as it provides a mild buffering environment that helps maintain RNA stability during experiments. The presence of EDTA chelates divalent cations, which can otherwise degrade RNA, ensuring better outcomes in gel electrophoresis and other molecular biology techniques. This makes it an excellent choice for researchers working with RNA isolation, purification, and characterization. -
Pergunta:
What are the benefits of using TAE over TBE buffers?
Resposta: TAE buffers are generally preferred over TBE for certain applications due to their gentler nature, which can produce less DNA smearing. TAE is less likely to inhibit enzymatic reactions, making it ideal when nucleic acids need to be recovered after electrophoresis. As a result, if you're looking to analyze DNA fragments or recover purified products efficiently, choosing TAE 10X can enhance your results in various molecular biology applications. -
Pergunta:
Can TAE 10X Solution be used multiple times?
Resposta: While TAE 10X Solution can be reused for multiple gel runs, its effectiveness may diminish over time due to the accumulation of salts and nucleic acids. It's advisable to filter the solution to remove any contaminants before reuse. In scenarios with high sample loading or when precise results are critical, preparing fresh TAE 1X Solution is recommended to ensure the accuracy and reliability of your gel electrophoresis results. -
Pergunta:
What safety precautions should be taken when handling TAE 10X Solution?
Resposta: When handling TAE 10X Solution, it is essential to wear appropriate personal protective equipment (PPE), including gloves and safety goggles, to prevent skin and eye contact. Ensure that you are working in a well-ventilated area to avoid inhalation of any aerosols. Proper lab safety protocols help protect you and ensure that the working environment remains safe, especially when using this solution in various molecular biology methodologies. -
Pergunta:
What is the shelf life of TAE 10X Solution?
Resposta: TAE 10X Solution has a shelf life of approximately one year when stored properly at room temperature and away from direct sunlight. For extended use, refrigerating the solution can increase its stability. Regularly checking for signs of contamination or precipitation is critical; if observed, it might be necessary to prepare a fresh solution to maintain experiment integrity. -
Pergunta:
How should TAE 10X Solution be stored?
Resposta: TAE 10X Solution should be stored in a cool, dry place, away from direct sunlight to maintain its effectiveness. Ideally, keeping it at room temperature or in a refrigerator prevents degradation of the components, maximizing its usability for upcoming experiments. Proper storage practices ensure that your experiments yield reliable and reproducible results, essential for any molecular biology work. -
Pergunta:
Can you use TAE 10X Solution for DNA sequencing?
Resposta: Yes, TAE 10X Solution can be used for DNA sequencing applications, particularly during gel electrophoresis steps to assess the size and purity of DNA fragments. Its buffering capacity maintains a neutral pH, critical for DNA stability during sequencing processes. Researchers can derive informative insights on fragment sizes, which supports successful sequencing and downstream analysis efforts in genetic research and diagnostics. -
Pergunta:
Where can I buy TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 Liters in Cabo Verde?
Resposta: You can purchase TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 Liters from Ubuy. Ubuy offers reliable services and a range of laboratory supplies that ensures you have access to the products you need for your molecular biology experiments. With easy navigation and efficient service, Ubuy simplifies the process of acquiring essential lab solutions.
RPI Buffers Editorial Review
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Prós
- High-quality formulation
- Reliable and consistent results
- Easy to use
- Great for various applications
- Well-reviewed by experts
Contras
- Some users prefer smaller sizes.
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CVE 17884
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Recursos e benefícios
- TRIS-Base: 0.4 M
- Acetic Acid: 0.2 M
- EDTA: 10 mM
- pH (10X Solution (@25°C): 8.2-8.4
- ISO 9001:2008 and GMP ISO 22716:2007 certified
- Not for food or drug use
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